Proteomic analysis of cytokine induced proteins in human intestinal epithelial cells: implications for inflammatory bowel diseases.

Details

Serval ID
serval:BIB_0CCACF3928B4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Proteomic analysis of cytokine induced proteins in human intestinal epithelial cells: implications for inflammatory bowel diseases.
Journal
Proteomics
Author(s)
Barceló-Batllori S., André M., Servis C., Lévy N., Takikawa O., Michetti P., Reymond M., Felley-Bosco E.
ISSN
1615-9853
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
2
Number
5
Pages
551-60
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
A role for cytokine regulated proteins in epithelial cells has been suggested in the pathogenesis of inflammatory bowel diseases (IBD). The aim of this study was to identify such cytokine regulated targets using a proteomic functional approach. Protein patterns from (35)S-radiolabeled homogenates of cultured colon epithelial cells were compared before and after exposure to interferon-gamma, interleukin-1beta and interleukin-6. Proteins were separated by two-dimensional polyacrylamide gel electrophoresis. Both autoradiographies and silver stained gels were analyzed. Proteins showing differential expression were identified by tryptic in-gel digestion and mass spectrometry. Metabolism related proteins were also investigated by Western blot analysis. Tryptophanyl-tRNA synthetase, indoleamine-2,3-dioxygenase, heterogeneous nuclear ribonucleoprotein JKTBP, interferon-induced 35kDa protein, proteasome subunit LMP2 and arginosuccinate synthetase were identified as cytokine modulated proteins in vitro. Using purified epithelial cells from patients, overexpression of indoleamine-2,3-dioxygenase, an enzyme involved in tryptophan metabolism, was confirmed in Crohn's disease as well as in ulcerative colitis, as compared to normal mucosa. No such difference was found in diverticulitis. Potentially, this observation opens new avenues in the treatment of IBD.
Keywords
Argininosuccinate Synthase, Cells, Cultured, Cytokines, Electrophoresis, Gel, Two-Dimensional, Epithelial Cells, Humans, Inflammatory Bowel Diseases, Intestinal Mucosa, Models, Biological, Proteome, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tryptophan Oxygenase, Tryptophan-tRNA Ligase
Pubmed
Web of science
Create date
25/01/2008 17:02
Last modification date
20/08/2019 13:34
Usage data