Climbing into their Skin to Understand Contextual Protein-Protein Associations and Localizations: Functional Investigations in Transgenic Live Model Organisms.
Détails
Télécharger: 38511872.pdf (1701.14 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY-NC-ND 4.0
Etat: Public
Version: Final published version
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_0739F71EFEB9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Climbing into their Skin to Understand Contextual Protein-Protein Associations and Localizations: Functional Investigations in Transgenic Live Model Organisms.
Périodique
Chembiochem
ISSN
1439-7633 (Electronic)
ISSN-L
1439-4227
Statut éditorial
Publié
Date de publication
16/04/2024
Peer-reviewed
Oui
Volume
25
Numéro
8
Pages
e202400005
Langue
anglais
Notes
Publication types: Journal Article ; Review
Publication Status: ppublish
Publication Status: ppublish
Résumé
Borrowing some quotes from Harper Lee's novel "To Kill A Mockingbird" to help frame our manuscript, we discuss methods to profile local proteomes. We initially focus on chemical biology regimens that function in live organisms and use reactive biotin species for this purpose. We then consider ways to add new dimensions to these experimental regimens, principally by releasing less reactive (i. e., more selective) (preter)natural electrophiles. Although electrophile release methods may have lower resolution and label fewer proteins than biotinylation methods, their ability to probe simultaneously protein function and locale raises new and interesting possibilities for the field.
Mots-clé
Biotin, Proteome, Biotinylation, APEX, Bio-ID and variants, Electrophile signaling, Function-guided spatial-proteomics, Protein-protein interactions, Proximity/interactome mapping tools, REX-technologies
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/03/2024 11:56
Dernière modification de la notice
23/04/2024 7:08