Rat liver 11 beta-hydroxysteroid dehydrogenase complementary deoxyribonucleic acid encodes oxoreductase activity in a mineralocorticoid-responsive toad bladder cell line

Détails

ID Serval
serval:BIB_05F93C78111A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Rat liver 11 beta-hydroxysteroid dehydrogenase complementary deoxyribonucleic acid encodes oxoreductase activity in a mineralocorticoid-responsive toad bladder cell line
Périodique
Endocrinology
Auteur⸱e⸱s
Duperrex  H., Kenouch  S., Gaeggeler  H. P., Seckl  J. R., Edwards  C. R., Farman  N., Rossier  B. C.
ISSN
0013-7227
Statut éditorial
Publié
Date de publication
02/1993
Peer-reviewed
Oui
Volume
132
Numéro
2
Pages
612-9
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
The mineralocorticoid receptor displays equal affinity for aldosterone and corticosterone. It has been proposed that aldosterone selectivity in vivo is achieved by the conversion of corticosterone into its inactive metabolite 11-dehydrocorticosterone by 11 beta-hydroxysteroid dehydrogenase (11 beta HSD). To test this hypothesis, we transfected rat liver 11 beta HSD cDNA into TBM cells, a sodium-transporting cell line. These cells respond equally well to aldosterone and corticosterone, indicating that endogenous 11 beta HSD is expressed at low levels in TBM cells. Although exogenous rat liver 11 beta HSD was expressed at high levels in transfected cells, mineralocorticoid selectivity was not observed. By contrast, the biologically inactive 11-dehydrocorticosterone was readily converted into corticosterone, a potent agonist for sodium transport. Our results indicate that rat liver 11 beta HSD behaves predominantly as a reductase in TBM cells. Another 11 beta HSD isoform is likely to be responsible for the dehydrogenase reaction in aldosterone-responsive cells.
Mots-clé
11-beta-Hydroxysteroid Dehydrogenases Animals Cell Line Corticosterone/*analogs & derivatives/metabolism/*pharmacology Dexamethasone/pharmacology Epithelium/enzymology/physiology Female Hydroxysteroid Dehydrogenases/*genetics/*metabolism Kidney/*metabolism Kinetics Liver/*enzymology Plasmids Rats Time Factors *Transfection Urinary Bladder/*drug effects/*enzymology
Pubmed
Web of science
Création de la notice
24/01/2008 14:00
Dernière modification de la notice
20/08/2019 13:28
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