Rat liver 11 beta-hydroxysteroid dehydrogenase complementary deoxyribonucleic acid encodes oxoreductase activity in a mineralocorticoid-responsive toad bladder cell line

Details

Serval ID
serval:BIB_05F93C78111A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rat liver 11 beta-hydroxysteroid dehydrogenase complementary deoxyribonucleic acid encodes oxoreductase activity in a mineralocorticoid-responsive toad bladder cell line
Journal
Endocrinology
Author(s)
Duperrex  H., Kenouch  S., Gaeggeler  H. P., Seckl  J. R., Edwards  C. R., Farman  N., Rossier  B. C.
ISSN
0013-7227
Publication state
Published
Issued date
02/1993
Peer-reviewed
Oui
Volume
132
Number
2
Pages
612-9
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
The mineralocorticoid receptor displays equal affinity for aldosterone and corticosterone. It has been proposed that aldosterone selectivity in vivo is achieved by the conversion of corticosterone into its inactive metabolite 11-dehydrocorticosterone by 11 beta-hydroxysteroid dehydrogenase (11 beta HSD). To test this hypothesis, we transfected rat liver 11 beta HSD cDNA into TBM cells, a sodium-transporting cell line. These cells respond equally well to aldosterone and corticosterone, indicating that endogenous 11 beta HSD is expressed at low levels in TBM cells. Although exogenous rat liver 11 beta HSD was expressed at high levels in transfected cells, mineralocorticoid selectivity was not observed. By contrast, the biologically inactive 11-dehydrocorticosterone was readily converted into corticosterone, a potent agonist for sodium transport. Our results indicate that rat liver 11 beta HSD behaves predominantly as a reductase in TBM cells. Another 11 beta HSD isoform is likely to be responsible for the dehydrogenase reaction in aldosterone-responsive cells.
Keywords
11-beta-Hydroxysteroid Dehydrogenases Animals Cell Line Corticosterone/*analogs & derivatives/metabolism/*pharmacology Dexamethasone/pharmacology Epithelium/enzymology/physiology Female Hydroxysteroid Dehydrogenases/*genetics/*metabolism Kidney/*metabolism Kinetics Liver/*enzymology Plasmids Rats Time Factors *Transfection Urinary Bladder/*drug effects/*enzymology
Pubmed
Web of science
Create date
24/01/2008 13:00
Last modification date
20/08/2019 12:28
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