High-performance liquid chromatographic assay with diode-array detection for toxicological screening of zopiclone, zolpidem, suriclone and alpidem in human plasma.

Détails

ID Serval
serval:BIB_0535BB7EFAA8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
High-performance liquid chromatographic assay with diode-array detection for toxicological screening of zopiclone, zolpidem, suriclone and alpidem in human plasma.
Périodique
Journal of Chromatography
Auteur⸱e⸱s
Tracqui A., Kintz P., Mangin P.
ISSN
0021-9673[print], 0021-9673[linking]
Statut éditorial
Publié
Date de publication
1993
Volume
616
Numéro
1
Pages
95-103
Langue
anglais
Résumé
A high-performance liquid chromatographic assay with diode-array detection has been developed for the toxicological screening of the newly developed non-benzodiazepine hypnotics and anxiolytics zopiclone, zolpidem, suriclone and alpidem. After single-step liquid-liquid extraction of plasma at pH 9.5 using chloroform-2-propanol-n-heptane (60:14:26, v/v), the substances are separated on a Nova-Pak C18 4-microns column (300 mm x 3.9 mm, I.D.), with methanol-tetrahydrofuran-pH 2.6 phosphate buffer (65:5:30, v/v) as the mobile phase (flow-rate 0.8 ml/min). Full ultraviolet spectra from 200 to 400 nm are recorded on-line during the entire analysis and may be automatically compared to spectra stored in a library. The retention times of the four drugs are 4.05 min (zopiclone), 4.66 min (zolpidem), 6.74 min (suriclone) and 10.97 min (alpidem). The analysis is performed in 15 min. The method is simple, rapid and highly specific. It is the first assay to be described for convenient screening of cyclopyrrolones and imidazopyridines.
Mots-clé
Adult, Anti-Anxiety Agents/blood, Anti-Anxiety Agents/poisoning, Azabicyclo Compounds, Chromatography, High Pressure Liquid, Female, Humans, Hydrogen-Ion Concentration, Hypnotics and Sedatives/blood, Hypnotics and Sedatives/poisoning, Imidazoles/blood, Imidazoles/poisoning, Piperazines/blood, Piperazines/poisoning, Pyridines/blood, Pyridines/poisoning, Spectrophotometry, Ultraviolet, Sulfur Compounds
Pubmed
Création de la notice
18/10/2010 15:08
Dernière modification de la notice
20/08/2019 13:27
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