High-performance liquid chromatographic assay with diode-array detection for toxicological screening of zopiclone, zolpidem, suriclone and alpidem in human plasma.

Details

Serval ID
serval:BIB_0535BB7EFAA8
Type
Article: article from journal or magazin.
Collection
Publications
Title
High-performance liquid chromatographic assay with diode-array detection for toxicological screening of zopiclone, zolpidem, suriclone and alpidem in human plasma.
Journal
Journal of Chromatography
Author(s)
Tracqui A., Kintz P., Mangin P.
ISSN
0021-9673[print], 0021-9673[linking]
Publication state
Published
Issued date
1993
Volume
616
Number
1
Pages
95-103
Language
english
Abstract
A high-performance liquid chromatographic assay with diode-array detection has been developed for the toxicological screening of the newly developed non-benzodiazepine hypnotics and anxiolytics zopiclone, zolpidem, suriclone and alpidem. After single-step liquid-liquid extraction of plasma at pH 9.5 using chloroform-2-propanol-n-heptane (60:14:26, v/v), the substances are separated on a Nova-Pak C18 4-microns column (300 mm x 3.9 mm, I.D.), with methanol-tetrahydrofuran-pH 2.6 phosphate buffer (65:5:30, v/v) as the mobile phase (flow-rate 0.8 ml/min). Full ultraviolet spectra from 200 to 400 nm are recorded on-line during the entire analysis and may be automatically compared to spectra stored in a library. The retention times of the four drugs are 4.05 min (zopiclone), 4.66 min (zolpidem), 6.74 min (suriclone) and 10.97 min (alpidem). The analysis is performed in 15 min. The method is simple, rapid and highly specific. It is the first assay to be described for convenient screening of cyclopyrrolones and imidazopyridines.
Keywords
Adult, Anti-Anxiety Agents/blood, Anti-Anxiety Agents/poisoning, Azabicyclo Compounds, Chromatography, High Pressure Liquid, Female, Humans, Hydrogen-Ion Concentration, Hypnotics and Sedatives/blood, Hypnotics and Sedatives/poisoning, Imidazoles/blood, Imidazoles/poisoning, Piperazines/blood, Piperazines/poisoning, Pyridines/blood, Pyridines/poisoning, Spectrophotometry, Ultraviolet, Sulfur Compounds
Pubmed
Create date
18/10/2010 14:08
Last modification date
20/08/2019 12:27
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