Localized in vivo 1H NMR detection of neurotransmitter labeling in rat brain during infusion of [1-13C] D-glucose.

Détails

ID Serval
serval:BIB_02A4767A9E77
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Localized in vivo 1H NMR detection of neurotransmitter labeling in rat brain during infusion of [1-13C] D-glucose.
Périodique
Magnetic Resonance in Medicine
Auteur⸱e⸱s
Pfeuffer J., Tkác I., Choi I.Y., Merkle H., Ugurbil K., Garwood M., Gruetter R.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Statut éditorial
Publié
Date de publication
1999
Volume
41
Numéro
6
Pages
1077-1083
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Résumé
Resolved localized nuclear magnetic resonance (NMR) signals of 1H bound to 13C label in the carbon positions of glutamate C4, C3 and glutamine C4, C3, as well as in aspartate C3, lactate C3, alanine C3, gamma-aminobutyric acid C3, and glucose C1 were simultaneously observed in spectra obtained from rat brain in vivo. Time-resolved label incorporation was measured with a new adiabatic carbon editing and decoupling (ACED) single-voxel stimulated echo acquisition mode (STEAM) sequence. Adiabatic carbon broadband decoupling of 12 kHz bandwidth was achieved in vivo, which decoupled the entire 13C spectrum at 9.4 T. Resonances from N-acetyl-aspartate and creatine were also detected, consistent with natural-abundance 13C levels. These results emphasize the potential of 1H NMR for following complex biochemical pathways in localized areas of resting rat brain as well as during focal activation using infusions of 13C-labeled glucose.
Mots-clé
Animals, Brain/metabolism, Brain Chemistry, Glucose/diagnostic use, Glutamine/analysis, Magnetic Resonance Spectroscopy/diagnostic use, Male, Neurotransmitter Agents/analysis, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Création de la notice
04/08/2010 15:28
Dernière modification de la notice
20/08/2019 12:24
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