Localized in vivo 1H NMR detection of neurotransmitter labeling in rat brain during infusion of [1-13C] D-glucose.

Details

Serval ID
serval:BIB_02A4767A9E77
Type
Article: article from journal or magazin.
Collection
Publications
Title
Localized in vivo 1H NMR detection of neurotransmitter labeling in rat brain during infusion of [1-13C] D-glucose.
Journal
Magnetic Resonance in Medicine
Author(s)
Pfeuffer J., Tkác I., Choi I.Y., Merkle H., Ugurbil K., Garwood M., Gruetter R.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Publication state
Published
Issued date
1999
Volume
41
Number
6
Pages
1077-1083
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Resolved localized nuclear magnetic resonance (NMR) signals of 1H bound to 13C label in the carbon positions of glutamate C4, C3 and glutamine C4, C3, as well as in aspartate C3, lactate C3, alanine C3, gamma-aminobutyric acid C3, and glucose C1 were simultaneously observed in spectra obtained from rat brain in vivo. Time-resolved label incorporation was measured with a new adiabatic carbon editing and decoupling (ACED) single-voxel stimulated echo acquisition mode (STEAM) sequence. Adiabatic carbon broadband decoupling of 12 kHz bandwidth was achieved in vivo, which decoupled the entire 13C spectrum at 9.4 T. Resonances from N-acetyl-aspartate and creatine were also detected, consistent with natural-abundance 13C levels. These results emphasize the potential of 1H NMR for following complex biochemical pathways in localized areas of resting rat brain as well as during focal activation using infusions of 13C-labeled glucose.
Keywords
Animals, Brain/metabolism, Brain Chemistry, Glucose/diagnostic use, Glutamine/analysis, Magnetic Resonance Spectroscopy/diagnostic use, Male, Neurotransmitter Agents/analysis, Rats, Rats, Sprague-Dawley
Pubmed
Web of science
Create date
04/08/2010 15:28
Last modification date
20/08/2019 12:24
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