Heterogeneity within the alkane-inducible cytochrome P450 gene family of the yeast Candida tropicalis

Détails

ID Serval
serval:BIB_0283D3DCC267
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Heterogeneity within the alkane-inducible cytochrome P450 gene family of the yeast Candida tropicalis
Périodique
FEBS Letters
Auteur⸱e⸱s
Sanglard  D., Fiechter  A.
ISSN
0014-5793 (Print)
Statut éditorial
Publié
Date de publication
10/1989
Volume
256
Numéro
1-2
Pages
128-34
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 9
Résumé
The reexamination of a genomic lambda gt11 Candida tropicalis expression library for the presence of genes related to the previously reported alkane-inducible cytochrome P450alk gene (P450alk), which is the first member of the P450LII gene family, was undertaken. A positive clone with a DNA fragment having 69% similarity with a portion of P450alk was isolated. As in the case of P450alk, this new putative P450 gene was also induced by tetradecane when C. tropicalis was grown on this carbon source and was therefore named P450alk2, P450alk1 corresponding to the first isolated P450 gene. In addition to P450alk2, the existence of other P450alk-related genes is suggested by the hybridization pattern of P450alk1 and P450alk2 probes with the C. tropicalis genomic DNA. The P450LII gene family in C. tropicalis appears therefore to include several different members. This heterogeneity is presently a unique feature within yeast P450 gene families and resembles the situation existing in P450 gene families of higher eukaryotes.
Mots-clé
Alkanes/*pharmacology Blotting, Southern Candida/drug effects/*genetics Cloning, Molecular Cytochrome P-450 Enzyme System/*genetics/isolation & purification DNA/isolation & purification Enzyme Induction/drug effects *Genes, Fungal Isoenzymes/*genetics Molecular Sequence Data Nucleic Acid Hybridization Plasmids Sequence Homology, Nucleic Acid
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 14:40
Dernière modification de la notice
20/08/2019 12:24
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