Heterogeneity within the alkane-inducible cytochrome P450 gene family of the yeast Candida tropicalis

Details

Serval ID
serval:BIB_0283D3DCC267
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Heterogeneity within the alkane-inducible cytochrome P450 gene family of the yeast Candida tropicalis
Journal
FEBS Letters
Author(s)
Sanglard  D., Fiechter  A.
ISSN
0014-5793 (Print)
Publication state
Published
Issued date
10/1989
Volume
256
Number
1-2
Pages
128-34
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 9
Abstract
The reexamination of a genomic lambda gt11 Candida tropicalis expression library for the presence of genes related to the previously reported alkane-inducible cytochrome P450alk gene (P450alk), which is the first member of the P450LII gene family, was undertaken. A positive clone with a DNA fragment having 69% similarity with a portion of P450alk was isolated. As in the case of P450alk, this new putative P450 gene was also induced by tetradecane when C. tropicalis was grown on this carbon source and was therefore named P450alk2, P450alk1 corresponding to the first isolated P450 gene. In addition to P450alk2, the existence of other P450alk-related genes is suggested by the hybridization pattern of P450alk1 and P450alk2 probes with the C. tropicalis genomic DNA. The P450LII gene family in C. tropicalis appears therefore to include several different members. This heterogeneity is presently a unique feature within yeast P450 gene families and resembles the situation existing in P450 gene families of higher eukaryotes.
Keywords
Alkanes/*pharmacology Blotting, Southern Candida/drug effects/*genetics Cloning, Molecular Cytochrome P-450 Enzyme System/*genetics/isolation & purification DNA/isolation & purification Enzyme Induction/drug effects *Genes, Fungal Isoenzymes/*genetics Molecular Sequence Data Nucleic Acid Hybridization Plasmids Sequence Homology, Nucleic Acid
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:40
Last modification date
20/08/2019 12:24
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