Genetic and physiological analysis of Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids

Détails

ID Serval
serval:BIB_016038A4482E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Genetic and physiological analysis of Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids
Périodique
Journal of Experimental Botany
Auteur⸱e⸱s
Gasperini D., Greenland A., Hedden P., Dreos R., Harwood W., Griffiths S.
ISSN
1460-2431 (Electronic)
ISSN-L
0022-0957
Statut éditorial
Publié
Date de publication
2012
Volume
63
Numéro
12
Pages
4419-4436
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Over the next decade, wheat grain production must increase to meet the demand of a fast growing human population. One strategy to meet this challenge is to raise wheat productivity by optimizing plant stature. The Reduced height 8 (Rht8) semi-dwarfing gene is one of the few, together with the Green Revolution genes, to reduce stature of wheat (Triticum aestivum L.), and improve lodging resistance, without compromising grain yield. Rht8 is widely used in dry environments such as Mediterranean countries where it increases plant adaptability. With recent climate change, its use could become increasingly important even in more northern latitudes. In the present study, the characterization of Rht8 was furthered. Morphological analyses show that the semi-dwarf phenotype of Rht8 lines is due to shorter internodal segments along the wheat culm, achieved through reduced cell elongation. Physiological experiments show that the reduced cell elongation is not due to defective gibberellin biosynthesis or signalling, but possibly to a reduced sensitivity to brassinosteroids. Using a fine-resolution mapping approach and screening 3104 F(2) individuals of a newly developed mapping population, the Rht8 genetic interval was reduced from 20.5 cM to 1.29 cM. Comparative genomics with model genomes confined the Rht8 syntenic intervals to 3.3 Mb of the short arm of rice chromosome 4, and to 2 Mb of Brachypodium distachyon chromosome 5. The very high resolution potential of the plant material generated is crucial for the eventual cloning of Rht8.
Mots-clé
Brachypodium/genetics, Brassinosteroids/pharmacology, Chromosomes, Plant/genetics, Genes, Plant/physiology, Gibberellins/metabolism, Oryza/genetics, Plant Growth Regulators/pharmacology, Plant Leaves/cytology, Plant Leaves/drug effects, Plant Leaves/genetics, Plant Leaves/physiology, Plant Roots/cytology, Plant Roots/drug effects, Plant Roots/genetics, Plant Roots/physiology, Seedlings/cytology, Seedlings/drug effects, Seedlings/genetics, Seedlings/physiology, Triticum/cytology, Triticum/drug effects, Triticum/genetics, Triticum/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/07/2018 9:00
Dernière modification de la notice
20/08/2019 12:23
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