Genetic and physiological analysis of Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids

Details

Serval ID
serval:BIB_016038A4482E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genetic and physiological analysis of Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids
Journal
Journal of Experimental Botany
Author(s)
Gasperini D., Greenland A., Hedden P., Dreos R., Harwood W., Griffiths S.
ISSN
1460-2431 (Electronic)
ISSN-L
0022-0957
Publication state
Published
Issued date
2012
Volume
63
Number
12
Pages
4419-4436
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Over the next decade, wheat grain production must increase to meet the demand of a fast growing human population. One strategy to meet this challenge is to raise wheat productivity by optimizing plant stature. The Reduced height 8 (Rht8) semi-dwarfing gene is one of the few, together with the Green Revolution genes, to reduce stature of wheat (Triticum aestivum L.), and improve lodging resistance, without compromising grain yield. Rht8 is widely used in dry environments such as Mediterranean countries where it increases plant adaptability. With recent climate change, its use could become increasingly important even in more northern latitudes. In the present study, the characterization of Rht8 was furthered. Morphological analyses show that the semi-dwarf phenotype of Rht8 lines is due to shorter internodal segments along the wheat culm, achieved through reduced cell elongation. Physiological experiments show that the reduced cell elongation is not due to defective gibberellin biosynthesis or signalling, but possibly to a reduced sensitivity to brassinosteroids. Using a fine-resolution mapping approach and screening 3104 F(2) individuals of a newly developed mapping population, the Rht8 genetic interval was reduced from 20.5 cM to 1.29 cM. Comparative genomics with model genomes confined the Rht8 syntenic intervals to 3.3 Mb of the short arm of rice chromosome 4, and to 2 Mb of Brachypodium distachyon chromosome 5. The very high resolution potential of the plant material generated is crucial for the eventual cloning of Rht8.
Keywords
Brachypodium/genetics, Brassinosteroids/pharmacology, Chromosomes, Plant/genetics, Genes, Plant/physiology, Gibberellins/metabolism, Oryza/genetics, Plant Growth Regulators/pharmacology, Plant Leaves/cytology, Plant Leaves/drug effects, Plant Leaves/genetics, Plant Leaves/physiology, Plant Roots/cytology, Plant Roots/drug effects, Plant Roots/genetics, Plant Roots/physiology, Seedlings/cytology, Seedlings/drug effects, Seedlings/genetics, Seedlings/physiology, Triticum/cytology, Triticum/drug effects, Triticum/genetics, Triticum/physiology
Pubmed
Web of science
Open Access
Yes
Create date
13/07/2018 10:00
Last modification date
20/08/2019 13:23
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