Melanoma patients respond to a cytotoxic T lymphocyte-defined self-peptide with diverse and nonoverlapping T-cell receptor repertoires.

Détails

ID Serval
serval:BIB_0128605EFE72
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Melanoma patients respond to a cytotoxic T lymphocyte-defined self-peptide with diverse and nonoverlapping T-cell receptor repertoires.
Périodique
Cancer research
Auteur⸱e⸱s
Dietrich P.Y., Walker P.R., Quiquerez A.L., Perrin G., Dutoit V., Liénard D., Guillaume P., Cerottini J.C., Romero P., Valmori D.
ISSN
0008-5472
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
61
Numéro
5
Pages
2047-54
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Résumé
HLA-A2+ melanoma patients develop naturally a strong CD8+ T cell response to a self-peptide derived from Melan-A. Here, we have used HLA-A2/peptide tetramers to isolate Melan-A-specific T cells from tumor-infiltrated lymph nodes of two HLA-A2+ melanoma patients and analyzed their TCR beta chain V segment and complementarity determining region 3 length and sequence. We found a broad diversity in Melan-A-specific immune T-cell receptor (TCR) repertoires in terms of both TCR beta chain variable gene segment usage and clonal composition. In addition, immune TCR repertoires selected in the patients were not overlapping. In contrast to previously characterized CD8+ T-cell responses to viral infections, this study provides evidence against usage of highly restricted TCR repertoire in the natural response to a self-differentiation tumor antigen.
Mots-clé
Amino Acid Sequence, Antigens, Neoplasm, Base Sequence, Complementarity Determining Regions, Epitopes, T-Lymphocyte, HLA-A2 Antigen, Humans, Immunodominant Epitopes, Immunoglobulin Variable Region, Lymph Nodes, Melanoma, Molecular Sequence Data, Neoplasm Proteins, Peptide Fragments, Receptors, Antigen, T-Cell, alpha-beta, T-Lymphocytes, Cytotoxic
Pubmed
Web of science
Création de la notice
28/01/2008 11:13
Dernière modification de la notice
20/08/2019 12:23
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