Melanoma patients respond to a cytotoxic T lymphocyte-defined self-peptide with diverse and nonoverlapping T-cell receptor repertoires.

Details

Serval ID
serval:BIB_0128605EFE72
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Melanoma patients respond to a cytotoxic T lymphocyte-defined self-peptide with diverse and nonoverlapping T-cell receptor repertoires.
Journal
Cancer research
Author(s)
Dietrich P.Y., Walker P.R., Quiquerez A.L., Perrin G., Dutoit V., Liénard D., Guillaume P., Cerottini J.C., Romero P., Valmori D.
ISSN
0008-5472
Publication state
Published
Issued date
2001
Peer-reviewed
Oui
Volume
61
Number
5
Pages
2047-54
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't - Publication Status: ppublish
Abstract
HLA-A2+ melanoma patients develop naturally a strong CD8+ T cell response to a self-peptide derived from Melan-A. Here, we have used HLA-A2/peptide tetramers to isolate Melan-A-specific T cells from tumor-infiltrated lymph nodes of two HLA-A2+ melanoma patients and analyzed their TCR beta chain V segment and complementarity determining region 3 length and sequence. We found a broad diversity in Melan-A-specific immune T-cell receptor (TCR) repertoires in terms of both TCR beta chain variable gene segment usage and clonal composition. In addition, immune TCR repertoires selected in the patients were not overlapping. In contrast to previously characterized CD8+ T-cell responses to viral infections, this study provides evidence against usage of highly restricted TCR repertoire in the natural response to a self-differentiation tumor antigen.
Keywords
Amino Acid Sequence, Antigens, Neoplasm, Base Sequence, Complementarity Determining Regions, Epitopes, T-Lymphocyte, HLA-A2 Antigen, Humans, Immunodominant Epitopes, Immunoglobulin Variable Region, Lymph Nodes, Melanoma, Molecular Sequence Data, Neoplasm Proteins, Peptide Fragments, Receptors, Antigen, T-Cell, alpha-beta, T-Lymphocytes, Cytotoxic
Pubmed
Web of science
Create date
28/01/2008 11:13
Last modification date
20/08/2019 12:23
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