The human fungal pathogen Candida glabrata is less virulent compared to the most isolated Candida species including Candida albicans. Its reduced metabolic flexibility, haploidy, and auxotrophies contribute to a "stealth and evasion" infection strategy, resulting in minimal tissue damage in the host. C. glabrata seems to be optimally adapted to infection of the urinary tract (UTI), especially in catheterized patients. UTIs are not well studied and often difficult to treat, given that not all antifungals penetrate in the bladder and that treatments through the catheter are not always possible since maintained catheterization increases the infection risk.In the recent effort to reduce the amount of animals used during scientific experiments, bioluminescence imaging is a useful tool. In this protocol, C. glabrata urinary tract infections were imaged in mice, thus facilitating the testing of possible new antifungals and novel treatment strategies.