Using Bioluminescence to Image Candida glabrata Urinary Tract Infections in Mice

Details

Ressource 1Request a copy Under indefinite embargo.
UNIL restricted access
State: Public
Version: author
License: Not specified
Serval ID
serval:BIB_005F9CC2A3CD
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
Using Bioluminescence to Image Candida glabrata Urinary Tract Infections in Mice
Title of the book
Antifungal Drug Resistance
Author(s)
Schrevens Sanne, Torelli Riccardo, Sanguinetti Maurizio, Sanglard Dominque
Publisher
Springer US
ISBN
9781071631546
9781071631553
Publication state
Published
Issued date
2023
Peer-reviewed
Oui
Volume
2658
Series
Methods in Molecular Biology
Pages
239-247
Language
english
Abstract
The human fungal pathogen Candida glabrata is less virulent compared to the most isolated Candida species including Candida albicans. Its reduced metabolic flexibility, haploidy, and auxotrophies contribute to a "stealth and evasion" infection strategy, resulting in minimal tissue damage in the host. C. glabrata seems to be optimally adapted to infection of the urinary tract (UTI), especially in catheterized patients. UTIs are not well studied and often difficult to treat, given that not all antifungals penetrate in the bladder and that treatments through the catheter are not always possible since maintained catheterization increases the infection risk.In the recent effort to reduce the amount of animals used during scientific experiments, bioluminescence imaging is a useful tool. In this protocol, C. glabrata urinary tract infections were imaged in mice, thus facilitating the testing of possible new antifungals and novel treatment strategies.
Keywords
Mice, Humans, Animals, Candida glabrata, Antifungal Agents/pharmacology, Antifungal Agents/therapeutic use, Candida, Candida albicans, Urinary Tract Infections/drug therapy, Urinary Tract Infections/microbiology, Bioluminescence, Urinary tract infection
Pubmed
Create date
11/04/2023 17:17
Last modification date
18/07/2024 7:07
Usage data