Circadian and feeding rhythms differentially affect rhythmic mRNA transcription and translation in mouse liver.

Details

Serval ID
serval:BIB_FFCC0CC29FFF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Circadian and feeding rhythms differentially affect rhythmic mRNA transcription and translation in mouse liver.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Atger F., Gobet C., Marquis J., Martin E., Wang J., Weger B., Lefebvre G., Descombes P., Naef F., Gachon F.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
112
Number
47
Pages
E6579-E6588
Language
english
Abstract
Diurnal oscillations of gene expression are a hallmark of rhythmic physiology across most living organisms. Such oscillations are controlled by the interplay between the circadian clock and feeding rhythms. Although rhythmic mRNA accumulation has been extensively studied, comparatively less is known about their transcription and translation. Here, we quantified simultaneously temporal transcription, accumulation, and translation of mouse liver mRNAs under physiological light-dark conditions and ad libitum or night-restricted feeding in WT and brain and muscle Arnt-like 1 (Bmal1)-deficient animals. We found that rhythmic transcription predominantly drives rhythmic mRNA accumulation and translation for a majority of genes. Comparison of wild-type and Bmal1 KO mice shows that circadian clock and feeding rhythms have broad impact on rhythmic gene expression, Bmal1 deletion affecting surprisingly both transcriptional and posttranscriptional levels. Translation efficiency is differentially regulated during the diurnal cycle for genes with 5'-Terminal Oligo Pyrimidine tract (5'-TOP) sequences and for genes involved in mitochondrial activity, many harboring a Translation Initiator of Short 5'-UTR (TISU) motif. The increased translation efficiency of 5'-TOP and TISU genes is mainly driven by feeding rhythms but Bmal1 deletion also affects amplitude and phase of translation, including TISU genes. Together this study emphasizes the complex interconnections between circadian and feeding rhythms at several steps ultimately determining rhythmic gene expression and translation.
Keywords
circadian rhythms, ribosome profiling, mRNA translation, 5 '-TOP sequences, TISU motifs
Pubmed
Web of science
Open Access
Yes
Create date
15/12/2015 17:29
Last modification date
17/09/2020 8:21
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