Human immunodeficiency virus (HIV) infection in CD4+ T lymphocytes genetically deficient in LFA-1: LFA-1 is required for HIV-mediated cell fusion but not for viral transmission.

Details

Serval ID
serval:BIB_FFB433AD725A
Type
Article: article from journal or magazin.
Collection
Publications
Title
Human immunodeficiency virus (HIV) infection in CD4+ T lymphocytes genetically deficient in LFA-1: LFA-1 is required for HIV-mediated cell fusion but not for viral transmission.
Journal
The Journal of experimental medicine
Author(s)
Pantaleo G., Butini L., Graziosi C., Poli G., Schnittman S.M., Greenhouse J.J., Gallin J.I., Fauci A.S.
ISSN
0022-1007 (Print)
ISSN-L
0022-1007
Publication state
Published
Issued date
01/02/1991
Peer-reviewed
Oui
Volume
173
Number
2
Pages
511-514
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
In the present study, we demonstrated that expression of the LFA-1 molecule is necessary for cell fusion and syncytia formation in human immunodeficiency virus (HIV)-infected CD4+ T lymphocytes. In contrast, the lack of expression of LFA-1 does not influence significantly cell-to-cell transmission of HIV. In fact, LFA-1- T lymphocytes obtained from a leukocyte adhesion deficiency patient were unable to fuse and form syncytia when infected with HIV-1 or HIV-2, despite the fact that efficiency of HIV infection (i.e., virus entry, HIV spreading, and levels of virus replication) was comparable with that observed in LFA-1+ T lymphocytes. In addition, we provide evidence that LFA-1 by mediating cell fusion contributes to the depletion of HIV-infected CD4+ T lymphocytes in vitro.
Keywords
Antibodies, Monoclonal, CD4-Positive T-Lymphocytes/microbiology, Cell Fusion, Cells, Cultured, Genes, Viral/genetics, Giant Cells, HIV-1/genetics, HIV-1/physiology, HIV-2/genetics, HIV-2/physiology, Humans, Lymphocyte Activation, Lymphocyte Function-Associated Antigen-1/deficiency, Lymphocyte Function-Associated Antigen-1/genetics, Lymphocyte Function-Associated Antigen-1/physiology, Phytohemagglutinins, Polymerase Chain Reaction, Virus Replication
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:58
Last modification date
01/07/2024 15:54
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