Connexin 43 expression in human hypertrophied heart due to pressure and volume overload.

Details

Serval ID
serval:BIB_FC61C3C4AD31
Type
Article: article from journal or magazin.
Collection
Publications
Title
Connexin 43 expression in human hypertrophied heart due to pressure and volume overload.
Journal
Physiological Research / Academia Scientiarum Bohemoslovaca
Author(s)
Vetter C., Zweifel M., Zuppinger C., Carrel T., Martin D., Haefliger J.A., Delacrétaz E.
ISSN
0862-8408[print], 0862-8408[linking]
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
59
Number
1
Pages
35-42
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Abstract
Left ventricular hypertrophy (LVH) is due to pressure overload or mechanical stretch and is thought to be associated with remodeling of gap-junctions. We investigated whether the expression of connexin 43 (Cx43) is altered in humans in response to different degrees of LVH. The expression of Cx43 was analyzed by quantitative polymerase chain reaction, Western blot analysis and immunohistochemistry on left ventricular biopsies from patients undergoing aortic or mitral valve replacement. Three groups were analyzed: patients with aortic stenosis with severe LVH (n=9) versus only mild LVH (n=7), and patients with LVH caused by mitral regurgitation (n=5). Cx43 mRNA expression and protein expression were similar in the three groups studied. Furthermore, immunohistochemistry revealed no change in Cx43 distribution. We can conclude that when compared with mild LVH or with LVH due to volume overload, severe LVH due to chronic pressure overload is not accompanied by detectable changes of Cx43 expression or spatial distribution.
Keywords
Aged, Aged, 80 and over, Aortic Valve Stenosis/complications, Aortic Valve Stenosis/metabolism, Biopsy, Blood Pressure, Blotting, Western, Connexin 43/analysis, Connexin 43/genetics, Female, Gene Expression Regulation, Humans, Hypertrophy, Left Ventricular/etiology, Hypertrophy, Left Ventricular/mortality, Immunohistochemistry, Male, Middle Aged, Mitral Valve Insufficiency/complications, Mitral Valve Insufficiency/metabolism, Myocardium/chemistry, Myocardium/pathology, RNA, Messenger/analysis, Reverse Transcriptase Polymerase Chain Reaction, Severity of Illness Index, Ventricular Function, Left
Pubmed
Web of science
Create date
11/03/2011 13:07
Last modification date
20/08/2019 17:27
Usage data