Measurement of cerebral monoamine oxidase B activity using L-[11C]deprenyl and dynamic positron emission tomography.

Details

Serval ID
serval:BIB_FABB910407F6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Measurement of cerebral monoamine oxidase B activity using L-[11C]deprenyl and dynamic positron emission tomography.
Journal
Journal of Cerebral Blood Flow and Metabolism
Author(s)
Lammertsma A.A., Bench C.J., Price G.W., Cremer J.E., Luthra S.K., Turton D., Wood N.D., Frackowiak R.S.
ISSN
0271-678X (Print)
ISSN-L
0271-678X
Publication state
Published
Issued date
1991
Volume
11
Number
4
Pages
545-556
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
A tracer kinetic procedure was developed for the measurement of monoamine oxidase type B (MAO-B) activity using L-[11C]deprenyl and positron emission tomography (PET). The kinetic model consisted of two tissue compartments with irreversible binding to the second compartment (three rate constants). In addition, a blood volume component was included. Special attention was given to the accurate measurement of the plasma and whole blood input functions. The method was applied to the measurement of the dose-response curve of a reversible MAO-B inhibitor (Ro 19-6327). From the results, it followed that the rate constant for irreversible binding (k3) appeared to be a better index of MAO-B activity than the net influx constant Ki. Furthermore, regional analysis demonstrated that Ki, but not k3, was flow dependent. This implies that full kinetic analysis is required for an accurate assessment of MAO-B activity.
Keywords
Aged, Brain/enzymology, Female, Humans, Kinetics, Male, Middle Aged, Monoamine Oxidase/metabolism, Monoamine Oxidase Inhibitors/pharmacology, Picolinic Acids/pharmacology, Selegiline/metabolism, Tomography, Emission-Computed
Pubmed
Web of science
Create date
25/09/2011 16:56
Last modification date
20/08/2019 16:26
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