Monalysin, a novel ß-pore-forming toxin from the Drosophila pathogen Pseudomonas entomophila, contributes to host intestinal damage and lethality.

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Ressource 1Download: Opota-2011-Monalysin, a novel beta-pore-forming.pdf (2652.61 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_F9D33D94FDE7
Type
Article: article from journal or magazin.
Collection
Publications
Title
Monalysin, a novel ß-pore-forming toxin from the Drosophila pathogen Pseudomonas entomophila, contributes to host intestinal damage and lethality.
Journal
Plos Pathogens
Author(s)
Opota O., Vallet-Gély I., Vincentelli R., Kellenberger C., Iacovache I., Gonzalez M.R., Roussel A., van der Goot F.G., Lemaitre B.
ISSN
1553-7374 (Electronic)
ISSN-L
1553-7366
Publication state
Published
Issued date
01/09/2011
Volume
7
Number
9
Pages
e1002259
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Pseudomonas entomophila is an entomopathogenic bacterium that infects and kills Drosophila. P. entomophila pathogenicity is linked to its ability to cause irreversible damages to the Drosophila gut, preventing epithelium renewal and repair. Here we report the identification of a novel pore-forming toxin (PFT), Monalysin, which contributes to the virulence of P. entomophila against Drosophila. Our data show that Monalysin requires N-terminal cleavage to become fully active, forms oligomers in vitro, and induces pore-formation in artificial lipid membranes. The prediction of the secondary structure of the membrane-spanning domain indicates that Monalysin is a PFT of the ß-type. The expression of Monalysin is regulated by both the GacS/GacA two-component system and the Pvf regulator, two signaling systems that control P. entomophila pathogenicity. In addition, AprA, a metallo-protease secreted by P. entomophila, can induce the rapid cleavage of pro-Monalysin into its active form. Reduced cell death is observed upon infection with a mutant deficient in Monalysin production showing that Monalysin plays a role in P. entomophila ability to induce intestinal cell damages, which is consistent with its activity as a PFT. Our study together with the well-established action of Bacillus thuringiensis Cry toxins suggests that production of PFTs is a common strategy of entomopathogens to disrupt insect gut homeostasis.
Keywords
Animals, Bacterial Toxins/genetics, Bacterial Toxins/metabolism, Cell Line, Drosophila melanogaster, Gene Expression Regulation, Bacterial/physiology, Intestinal Diseases/genetics, Intestinal Diseases/metabolism, Intestines/metabolism, Intestines/microbiology, Lipid Bilayers/chemistry, Lipid Bilayers/metabolism, Pore Forming Cytotoxic Proteins/genetics, Pore Forming Cytotoxic Proteins/metabolism, Protein Structure, Tertiary, Pseudomonas/genetics, Pseudomonas/metabolism
Pubmed
Open Access
Yes
Create date
02/11/2015 17:59
Last modification date
21/08/2019 6:36
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