Derivation of traceable and transplantable photoreceptors from mouse embryonic stem cells.
Details
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State: Public
Version: Final published version
State: Public
Version: Final published version
Serval ID
serval:BIB_F919AF069BA0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Derivation of traceable and transplantable photoreceptors from mouse embryonic stem cells.
Journal
Stem Cell Reports
ISSN
2213-6711 (Electronic)
ISSN-L
2213-6711
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
2
Number
6
Pages
853-865
Language
english
Notes
Publication types: Journal ArticlePublication Status: epublish
Abstract
Retinal degenerative diseases resulting in the loss of photoreceptors are one of the major causes of blindness. Photoreceptor replacement therapy is a promising treatment because the transplantation of retina-derived photoreceptors can be applied now to different murine retinopathies to restore visual function. To have an unlimited source of photoreceptors, we derived a transgenic embryonic stem cell (ESC) line in which the Crx-GFP transgene is expressed in photoreceptors and assessed the capacity of a 3D culture protocol to produce integration-competent photoreceptors. This culture system allows the production of a large number of photoreceptors recapitulating the in vivo development. After transplantation, integrated cells showed the typical morphology of mature rods bearing external segments and ribbon synapses. We conclude that a 3D protocol coupled with ESCs provides a safe and renewable source of photoreceptors displaying a development and transplantation competence comparable to photoreceptors from age-matched retinas.
Pubmed
Web of science
Open Access
Yes
Create date
07/07/2014 8:48
Last modification date
20/08/2019 17:24