Long synthetic peptides as biologically active proteins: the example of the chemokines.

Details

Serval ID
serval:BIB_F8C31B6E07F2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Long synthetic peptides as biologically active proteins: the example of the chemokines.
Journal
Biologicals : Journal of the International Association of Biological Standardization
Author(s)
Thierry A.C., Pinaud S., Bigler N., Perrenoud G., Denis B., Roggero M.A., Fasel N., Moulon C., Demotz S.
ISSN
1045-1056 (Print)
ISSN-L
1045-1056
Publication state
Published
Issued date
2001
Volume
29
Number
3-4
Pages
259-263
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Chemokines constitute an expanding protein family of over 40 members which exhibit a wide variety of biological activities and are involved in many normal physiological processes, such as cellular migration, differentiation and activation, but also in pathological situations, such as inflammation and metastasis. Over the last few years, we have developed methods to manufacture long synthetic peptides of up to 130 residues, and to achieve the formation of native-like cysteine pairings. This ability prompted us to undertake the total chemical synthesis of chemokines. So far, we have successfully produced over 30 chemokine species, which exhibit biological activities similar to, or greater than, those reported by others. Chemical synthesis offers a clear advantage over recombinant technologies for the introduction of fluorochromes and haptens at molecularly defined positions. In addition, approval of chemically synthesized products for use in humans is straightforward compared with material produced by biological methods.
Keywords
Chemokines/chemistry, Chemokines/isolation & purification, Chromatography, High Pressure Liquid, Humans, Peptides/chemistry, Peptides/isolation & purification
Pubmed
Web of science
Create date
24/01/2008 15:02
Last modification date
20/08/2019 16:24
Usage data