Dextran sulphate reduces diphenylhexatriene anisotropy in human peripheral blood lymphocytes: impact on plasma membrane fluidity and HIV-cytopathogenicity.

Details

Serval ID
serval:BIB_F7F698C76197
Type
Article: article from journal or magazin.
Collection
Publications
Title
Dextran sulphate reduces diphenylhexatriene anisotropy in human peripheral blood lymphocytes: impact on plasma membrane fluidity and HIV-cytopathogenicity.
Journal
Journal of Antimicrobial Chemotherapy
Author(s)
Lehr H.A., Zimmer J.P., Hübner C., Reisinger E.C., Kohlschütter A., Schmitz H.
ISSN
0305-7453 (Print)
ISSN-L
0305-7453
Publication state
Published
Issued date
1991
Volume
28
Number
5
Pages
677-680
Language
english
Abstract
Cytopathogenicity of HIV and other enveloped viruses is reduced by membrane fluidizing agents and by dextran sulphate (DS). To investigate whether DS exerts its antiviral action via plasma membrane fluidization of host cells, we performed anisotropy measurements on human peripheral blood lymphocytes (PBL) using the fluorescent marker diphenylhexatriene. Anisotropy was decreased in DS-exposed PBL indicating increased fluidity in the hydrophobic membrane interior.
Keywords
Adult, Antigens, CD4/immunology, Cell Membrane/drug effects, Cell Membrane/physiology, Cytopathogenic Effect, Viral/drug effects, Dextran Sulfate/pharmacology, Diphenylhexatriene/diagnostic use, Drug Synergism, Female, HIV-1/drug effects, HIV-1/pathogenicity, Humans, Lymphocytes/drug effects, Lymphocytes/immunology, Male, Membrane Fluidity/drug effects, Membrane Fluidity/physiology, Spectrometry, Fluorescence, Trypan Blue
Pubmed
Create date
25/11/2011 18:42
Last modification date
20/08/2019 16:24
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