Article: article from journal or magazin.
Dextran sulphate reduces diphenylhexatriene anisotropy in human peripheral blood lymphocytes: impact on plasma membrane fluidity and HIV-cytopathogenicity.
Journal of Antimicrobial Chemotherapy
Cytopathogenicity of HIV and other enveloped viruses is reduced by membrane fluidizing agents and by dextran sulphate (DS). To investigate whether DS exerts its antiviral action via plasma membrane fluidization of host cells, we performed anisotropy measurements on human peripheral blood lymphocytes (PBL) using the fluorescent marker diphenylhexatriene. Anisotropy was decreased in DS-exposed PBL indicating increased fluidity in the hydrophobic membrane interior.
Adult, Antigens, CD4/immunology, Cell Membrane/drug effects, Cell Membrane/physiology, Cytopathogenic Effect, Viral/drug effects, Dextran Sulfate/pharmacology, Diphenylhexatriene/diagnostic use, Drug Synergism, Female, HIV-1/drug effects, HIV-1/pathogenicity, Humans, Lymphocytes/drug effects, Lymphocytes/immunology, Male, Membrane Fluidity/drug effects, Membrane Fluidity/physiology, Spectrometry, Fluorescence, Trypan Blue
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