Dextran sulphate reduces diphenylhexatriene anisotropy in human peripheral blood lymphocytes: impact on plasma membrane fluidity and HIV-cytopathogenicity.

Détails

ID Serval
serval:BIB_F7F698C76197
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Dextran sulphate reduces diphenylhexatriene anisotropy in human peripheral blood lymphocytes: impact on plasma membrane fluidity and HIV-cytopathogenicity.
Périodique
Journal of Antimicrobial Chemotherapy
Auteur(s)
Lehr H.A., Zimmer J.P., Hübner C., Reisinger E.C., Kohlschütter A., Schmitz H.
ISSN
0305-7453 (Print)
ISSN-L
0305-7453
Statut éditorial
Publié
Date de publication
1991
Volume
28
Numéro
5
Pages
677-680
Langue
anglais
Résumé
Cytopathogenicity of HIV and other enveloped viruses is reduced by membrane fluidizing agents and by dextran sulphate (DS). To investigate whether DS exerts its antiviral action via plasma membrane fluidization of host cells, we performed anisotropy measurements on human peripheral blood lymphocytes (PBL) using the fluorescent marker diphenylhexatriene. Anisotropy was decreased in DS-exposed PBL indicating increased fluidity in the hydrophobic membrane interior.
Mots-clé
Adult, Antigens, CD4/immunology, Cell Membrane/drug effects, Cell Membrane/physiology, Cytopathogenic Effect, Viral/drug effects, Dextran Sulfate/pharmacology, Diphenylhexatriene/diagnostic use, Drug Synergism, Female, HIV-1/drug effects, HIV-1/pathogenicity, Humans, Lymphocytes/drug effects, Lymphocytes/immunology, Male, Membrane Fluidity/drug effects, Membrane Fluidity/physiology, Spectrometry, Fluorescence, Trypan Blue
Pubmed
Création de la notice
25/11/2011 19:42
Dernière modification de la notice
03/03/2018 22:51
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