Transscleral Optical Phase Imaging of the Human Retina.

Details

Serval ID
serval:BIB_F1C5D31AEDAD
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Transscleral Optical Phase Imaging of the Human Retina.
Journal
Nature photonics
Author(s)
Laforest T., Künzi M., Kowalczuk L., Carpentras D., Behar-Cohen F., Moser C.
ISSN
1749-4885 (Print)
ISSN-L
1749-4885
Publication state
Published
Issued date
07/2020
Peer-reviewed
Oui
Volume
14
Number
7
Pages
439-445
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
In-vivo observation of the human retina at the cellular level is crucial to detect the first signs of retinal diseases and properly treat them. Despite the phenomenal advances in adaptive optics (AO) systems, clinical imaging of many retinal cells is still elusive due to the low signal-to-noise ratio induced by transpupillary illumination. We present a transscleral optical phase imaging (TOPI) method, which relies on high-angle oblique illumination of the retina, combined with AO, to enhance cell contrast. Examination of eleven healthy volunteer eyes, without pupil dilation, shows the ability of this method to produce in-vivo images of retinal cells, from the retinal pigment epithelium to the nerve fibre layer. This method also allows the generation of high-resolution label-free ex-vivo phase images of flat-mounted retinas. The 4.4°x 4.4° field-of-view in-vivo images are recorded in less than 10 seconds, opening new avenues in the exploration of healthy and diseased retinas.
Keywords
Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials
Pubmed
Web of science
Funding(s)
Other / EIT Health_ASSESS
Create date
05/05/2020 9:54
Last modification date
21/01/2021 7:26
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