Embryonic gene expression of Coregonus palaea (whitefish) under pathogen stress as analyzed by high-throughput RNA-sequencing.

Details

Serval ID
serval:BIB_EF118418A898
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Embryonic gene expression of Coregonus palaea (whitefish) under pathogen stress as analyzed by high-throughput RNA-sequencing.
Journal
Fish and Shellfish Immunology
Author(s)
Wilkins L.G., Clark E.S., Farinelli L., Wedekind C., Fumagalli L.
ISSN
1095-9947 (Electronic)
ISSN-L
1050-4648
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
47
Number
1
Pages
130-140
Language
english
Abstract
Most fishes produce free-living embryos that are exposed to environmental stressors immediately following fertilization, including pathogenic microorganisms. Initial immune protection of embryos involves the chorion, as a protective barrier, and maternally-allocated antimicrobial compounds. At later developmental stages, host-genetic effects influence susceptibility and tolerance, suggesting a direct interaction between embryo genes and pathogens. So far, only a few host genes could be identified that correlate with embryonic survival under pathogen stress in salmonids. Here, we utilized high-throughput RNA-sequencing in order to describe the transcriptional response of a non-model fish, the Alpine whitefish Coregonus palaea, to infection, both in terms of host genes that are likely manipulated by the pathogen, and those involved in an early putative immune response. Embryos were produced in vitro, raised individually, and exposed at the late-eyed stage to a virulent strain of the opportunistic fish pathogen Pseudomonas fluorescens. The pseudomonad increased embryonic mortality and affected gene expression substantially. For example, essential, upregulated metabolic pathways in embryos under pathogen stress included ion binding pathways, aminoacyl-tRNA-biosynthesis, and the production of arginine and proline, most probably mediated by the pathogen for its proliferation. Most prominently downregulated transcripts comprised the biosynthesis of unsaturated fatty acids, the citrate cycle, and various isoforms of b-cell transcription factors. These factors have been shown to play a significant role in host blood cell differentiation and renewal. With regard to specific immune functions, differentially expressed transcripts mapped to the complement cascade, MHC class I and II, TNF-alpha, and T-cell differentiation proteins. The results of this study reveal insights into how P. fluorescens impairs the development of whitefish embryos and set a foundation for future studies investigating host pathogen interactions in fish embryos.
Keywords
Adaptive Immunity, Animals, Cytokines/genetics, Cytokines/metabolism, Embryo, Nonmammalian/immunology, Embryo, Nonmammalian/metabolism, Fish Diseases/genetics, Fish Diseases/immunology, Fish Proteins/genetics, Fish Proteins/metabolism, Gene Expression Regulation, High-Throughput Nucleotide Sequencing/veterinary, Pseudomonas Infections/genetics, Pseudomonas Infections/immunology, Pseudomonas fluorescens/physiology, Salmonidae, Sequence Analysis, DNA, Transcriptome
Pubmed
Web of science
Create date
28/08/2015 13:32
Last modification date
20/08/2019 16:16
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