Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?

Details

Serval ID
serval:BIB_EEA03BB28207
Type
Article: article from journal or magazin.
Collection
Publications
Title
Mlx, a new Max-like bHLHZip family member: the center stage of a novel transcription factors regulatory pathway?
Journal
Oncogene
Author(s)
Meroni G., Cairo S., Merla G., Messali S., Brent R., Ballabio A., Reymond A.
ISSN
0950-9232 (Print)
ISSN-L
0950-9232
Publication state
Published
Issued date
2000
Volume
19
Number
29
Pages
3266-3277
Language
english
Abstract
The Myc proto-oncogene family members have been identified as the cellular homologs of the transforming oncogene of avian retroviruses. They encode central regulators of mammalian cell proliferation and apoptosis, and they associate with the bHLHZip protein Max to bind specific DNA sequences and regulate the expression of genes important for cell cycle progression. The other family members, Mad1, Mxi1, Mad3, Mad4 and Rox (Mnt) antagonize their activities. The Mads and Rox compete with Myc in heterodimerizing with Max and in binding to the same specific target sequences. These Mads:Max and Rox:Max dimers repress transcription through binding to the mSIN3 corepressor protein and by tethering histone deacetylase-containing complexes to the DNA. In a screen for Rox interactors we isolated Mlx, a bHLHZip protein previously identified in a screen for Mad1 interactors. In the present work we extend the known dimerization partners of Mlx by demonstrating its ability to interact with Rox. Moreover, we show that contrary to previous reports Mlx is able to homodimerize and to bind E-box sequences at low concentration levels. The possible role of Mlx in an emerging regulatory pathway and acting parallel to the Max driven network is discussed.
Keywords
Amino Acid Sequence, Animals, Base Sequence, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Basic-Leucine Zipper Transcription Factors, COS Cells, Cell Line, Transformed, DNA/metabolism, DNA, Complementary, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Dimerization, Gene Expression, Genes, Suppressor, Hela Cells, Helix-Loop-Helix Motifs, Humans, Leucine Zippers, Molecular Sequence Data, Nuclear Proteins/metabolism, Protein Isoforms/genetics, Protein Isoforms/metabolism, Rabbits, Repressor Proteins, Signal Transduction, Subcellular Fractions, Transcription Factors/genetics, Transcription Factors/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 15:51
Last modification date
20/08/2019 16:16
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