Accuracy profile validation of a new analytical method for butane measurement using headspace-gas chromatography-mass spectrometry.

Details

Serval ID
serval:BIB_EE873B8A0DD8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Accuracy profile validation of a new analytical method for butane measurement using headspace-gas chromatography-mass spectrometry.
Journal
Journal of Chromatography. B, Analytical Technologies In the Biomedical and Life Sciences
Author(s)
Varlet V., Smith F., Augsburger M.
ISSN
1873-376X (Electronic)
ISSN-L
1570-0232
Publication state
Published
Issued date
2013
Volume
913-914
Pages
155-160
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
The aim of our study was to provide an innovative HS-GC/MS method applicable to the routine determination of butane concentration in forensic toxicology laboratories. The main drawback of the GC/MS methods discussed in literature concerning butane measurement was the absence of a specific butane internal standard necessary to perform quantification. Because no stable isotope of butane is commercially available, it is essential to develop a new approach by an in situ generation of standards. To avoid the manipulation of a stable isotope-labelled gas, we have chosen to generate in situ an internal labelled standard gas (C(4)H(9)D) following the basis of the stoichiometric formation of butane by the reaction of deuterated water (D(2)O) with Grignard reagent butylmagnesium chloride (C(4)H(9)MgCl). This method allows a precise measurement of butane concentration and therefore, a full validation by accuracy profile was presented.
Keywords
In situ stable labelled isotope generation, Butane, Deuterated butane, Grignard reagent, Headspace gas extraction
Pubmed
Web of science
Create date
07/03/2013 19:29
Last modification date
20/08/2019 17:16
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