Colorectal poorly differentiated neuroendocrine carcinomas and mixed adenoneuroendocrine carcinomas: insights into the diagnostic immunophenotype, assessment of methylation profile, and search for prognostic markers.

Details

Serval ID
serval:BIB_ED64D00CF7D5
Type
Article: article from journal or magazin.
Collection
Publications
Title
Colorectal poorly differentiated neuroendocrine carcinomas and mixed adenoneuroendocrine carcinomas: insights into the diagnostic immunophenotype, assessment of methylation profile, and search for prognostic markers.
Journal
American Journal of Surgical Pathology
Author(s)
La Rosa S., Marando A., Furlan D., Sahnane N., Capella C.
ISSN
1532-0979 (Electronic)
ISSN-L
0147-5185
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
36
Number
4
Pages
601-611
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Colorectal poorly differentiated neuroendocrine carcinomas (NECs) and mixed adenoneuroendocrine carcinomas (MANECs) are well-recognized entities generally known to be associated with biological aggressiveness and poor patient survival. However, a few published papers have highlighted the existence of a subgroup of tumors with a better survival than expected; however, to date, there are no established parameters that usefully identify this category. In the present study we have investigated the morphologic features, the CpG methylator phenotype (CIMP), microsatellite instability (MSI), and the immunohistochemical profile, including the expression of transcription factors (TTF1, ASH1, CDX2, and PAX5), stem cell markers (CD117 and CD34), and cytokeratins 7 and 20, in a series of 39 carcinomas (27 NECs and 12 MANECs) to better characterize such neoplasms and to search for prognostic indicators. No different patient survival was observed between NECs and MANECs. Neoplasms showed a heterogenous spectrum of morphologic and immunohistochemical features; however, only large-cell subtype, significant peritumoral lymphoid reaction, CD117 immunoreactivity, vascular invasion, and MSI/CIMP+ status were significantly correlated with prognosis on univariable analysis. Furthermore, vascular invasion and CD117 immunoreactivity were independent prognostic markers on multivariable analysis. In addition to these prognostic features, neoplasms showed different expression of transcription factors, stem cell markers, and cytokeratins that should be considered for diagnostic purposes and, especially, for discriminating among possible differential diagnoses.
Keywords
Adenocarcinoma/diagnosis, Adenocarcinoma/genetics, Adult, Aged, Aged, 80 and over, Biomarkers, Tumor/metabolism, Carcinoma, Neuroendocrine/diagnosis, Carcinoma, Neuroendocrine/genetics, Colorectal Neoplasms/diagnosis, Colorectal Neoplasms/genetics, DNA Methylation, DNA, Neoplasm/genetics, Epigenesis, Genetic, Female, Humans, Immunophenotyping, Male, Microsatellite Instability, Middle Aged, Neoplasms, Multiple Primary, Prognosis, Proto-Oncogene Proteins c-kit/metabolism, Real-Time Polymerase Chain Reaction, Survival Rate
Pubmed
Web of science
Create date
06/09/2016 13:09
Last modification date
20/08/2019 16:15
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