Nanoneedle-Mediated Stimulation of Cell Mechanotransduction Machinery.

Details

Serval ID
serval:BIB_EC4BA54639FF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Nanoneedle-Mediated Stimulation of Cell Mechanotransduction Machinery.
Journal
ACS Nano
Author(s)
Hansel C.S., Crowder S.W., Cooper S., Gopal S., João Pardelha da Cruz M., de Oliveira Martins L., Keller D., Rothery S., Becce M., Cass AEG, Bakal C., Chiappini C., Stevens M.M.
ISSN
1936-086X (Electronic)
ISSN-L
1936-0851
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
13
Number
3
Pages
2913-2926
Language
english
Abstract
Biomaterial substrates can be engineered to present topographical signals to cells which, through interactions between the material and active components of the cell membrane, regulate key cellular processes and guide cell fate decisions. However, targeting mechanoresponsive elements that reside within the intracellular domain is a concept that has only recently emerged. Here, we show that mesoporous silicon nanoneedle arrays interact simultaneously with the cell membrane, cytoskeleton, and nucleus of primary human cells, generating distinct responses at each of these cellular compartments. Specifically, nanoneedles inhibit focal adhesion maturation at the membrane, reduce tension in the cytoskeleton, and lead to remodeling of the nuclear envelope at sites of impingement. The combined changes in actin cytoskeleton assembly, expression and segregation of the nuclear lamina, and localization of Yes-associated protein (YAP) correlate differently from what is canonically observed upon stimulation at the cell membrane, revealing that biophysical cues directed to the intracellular space can generate heretofore unobserved mechanosensory responses. These findings highlight the ability of nanoneedles to study and direct the phenotype of large cell populations simultaneously, through biophysical interactions with multiple mechanoresponsive components.
Keywords
cell−material interactions, mechanotransduction, nanoneedles, nuclear mechanics, porous silicon, super-resolution microscopy
Pubmed
Web of science
Open Access
Yes
Create date
18/04/2019 13:12
Last modification date
20/08/2019 17:14
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