Using BUSCO to Assess Insect Genomic Resources

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Version: Author's accepted manuscript
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Serval ID
serval:BIB_EBF3D80AC001
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
Using BUSCO to Assess Insect Genomic Resources
Title of the book
Methods in Molecular Biology
Author(s)
Waterhouse Robert M., Seppey Mathieu, Simão Felipe A., Zdobnov Evgeny M.
Publisher
Springer New York
Address of publication
New York
ISBN
9781493987740
9781493987757
ISSN
1064-3745
1940-6029
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Pages
59-74
Language
english
Abstract
The increasing affordability of sequencing technologies offers many new and exciting opportunities to address a diverse array of biological questions. This is evidenced in entomological research by numerous genomics and transcriptomics studies that attempt to decipher the often complex relationships amongst different species or orders and to build ‘omics’ resources to drive advancement of the molecular understanding of insect biology. Being able to gauge the quality of the sequencing data is of critical importance to understanding the potential limitations on the types of questions that these data can be reliably used to address. This chapter details the use of the Benchmarking Universal Single-Copy Orthologue (BUSCO) assessment tool to estimate the completeness of transcriptomes, genome assemblies, and annotated gene sets in terms of their expected gene content.
Keywords
Genomics, Transcriptomics, Genome annotation, Completeness assessment, Single - copy orthologues
Pubmed
Funding(s)
Swiss National Science Foundation / Careers / PP00P3_170664
Create date
12/11/2018 11:16
Last modification date
04/01/2020 7:17
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