Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results.

Details

Serval ID
serval:BIB_EB349E4D572A
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Institution
Title
Diagnostic strategies for SARS-CoV-2 infection and interpretation of microbiological results.
Journal
Clinical microbiology and infection
Author(s)
Caruana G., Croxatto A., Coste A.T., Opota O., Lamoth F., Jaton K., Greub G.
ISSN
1469-0691 (Electronic)
ISSN-L
1198-743X
Publication state
Published
Issued date
25/06/2020
Peer-reviewed
Oui
Language
english
Notes
Publication types: Journal Article ; Review
Publication Status: aheadofprint
Abstract
To face the current COVID-19 pandemic, diagnostic tools are essential. It is recommended to use real-time RT-PCR for RNA viruses in order (a) to perform a rapid and accurate diagnostic, (b) to guide patient care and management and (c) to guide epidemiological strategies. Further studies are warranted to define the role of serological diagnosis and a possible correlation between serological response and prognosis.
The aim was to guide clinical microbiologists in the use of these diagnostic tests and clinicians in the interpretation of their results.
A search of literature was performed through PubMed and Google Scholar using the keywords SARS-CoV-2, SARS-CoV-2 molecular diagnosis, SARS-CoV-2 immune response, SARS-CoV-2 serology/antibody testing, coronavirus diagnosis.
The present review discusses performances, limitations and use of current and future diagnostic tests for SARS-CoV-2.
Real-time RT-PCR remains the reference method for diagnosis of SARS-CoV-2 infection. On the other hand, notwithstanding its varying sensitivity according to the time of infection, serology represents a valid asset (a) to try to solve possible discrepancies between a highly suggestive clinical and radiological presentation and negative RT-PCR, (b) to solve discrepancies between different PCR assays and (c) for epidemiological purposes.
Keywords
COVID-19, Coronavirus, Molecular Diagnostic Testing, Reverse Transcriptase Polymerase Chain Reaction, SARS-COV-2, Serology, Molecular diagnostic testing, Reverse transcriptase polymerase chain reaction
Pubmed
Web of science
Open Access
Yes
Create date
03/07/2020 16:48
Last modification date
23/10/2020 5:26
Usage data