The histone-interacting domain of nuclear factor I activates simian virus 40 DNA replication in vivo.

Details

Serval ID
serval:BIB_EA36FD9A7818
Type
Article: article from journal or magazin.
Collection
Publications
Title
The histone-interacting domain of nuclear factor I activates simian virus 40 DNA replication in vivo.
Journal
Journal of Biological Chemistry
Author(s)
Müller K., Mermod N.
ISSN
0021-9258[print], 0021-9258[linking]
Publication state
Published
Issued date
2000
Volume
275
Number
3
Pages
1645-1650
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Efficient initiation of SV40 DNA replication requires transcription factors that bind auxiliary sequences flanking the minimally required origin. To evaluate the possibility that transcription factors may activate SV40 replication by acting on the chromatin structure of the origin, we used an in vivo replication system in which we targeted GAL4 fusion proteins to the minimally required origin. We found that the proline-rich transcriptional activation domain of nuclear factor I (NF-I), which has been previously shown to interact with histone H3, specifically activates replication. Evaluation of a series of deletion and point mutants of NF-I indicates that the H3-binding domain and the replication activity coincide perfectly. Assays with other transcription factors, such as Sp1, confirmed the correlation between the interaction with H3 and the activation of replication. These findings imply that transcription factors such as NF-I can activate SV40 replication via direct interaction with chromatin components, thereby contributing to the relief of nucleosomal repression at the SV40 origin.
Keywords
Animals, CCAAT-Enhancer-Binding Proteins, COS Cells, Carrier Proteins/metabolism, Chromatin/metabolism, DNA Replication, DNA, Viral/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Histones/metabolism, NFI Transcription Factors, Organic Cation Transport Proteins, Plasmids/metabolism, Point Mutation, Protein Structure, Tertiary, Simian virus 40/genetics, Simian virus 40/metabolism, Sp1 Transcription Factor/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Transcriptional Activation, Y-Box-Binding Protein 1
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 11:41
Last modification date
20/08/2019 17:12
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