The fusion protein of wild-type canine distemper virus is a major determinant of persistent infection.

Details

Serval ID
serval:BIB_E750637DDD09
Type
Article: article from journal or magazin.
Collection
Publications
Title
The fusion protein of wild-type canine distemper virus is a major determinant of persistent infection.
Journal
Virology
Author(s)
Plattet P., Rivals J.P., Zuber B., Brunner J.M., Zurbriggen A., Wittek R.
ISSN
0042-6822[print], 0042-6822[linking]
Publication state
Published
Issued date
07/2005
Volume
337
Number
2
Pages
312-326
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
The wild-type A75/17 canine distemper virus (CDV) strain induces a persistent infection in the central nervous system but infects cell lines very inefficiently. In contrast, the genetically more distant Onderstepoort CDV vaccine strain (OP-CDV) induces extensive syncytia formation. Here, we investigated the roles of wild-type fusion (F(WT)) and attachment (H(WT)) proteins in Vero cells expressing, or not, the canine SLAM receptor by transfection experiments and by studying recombinants viruses expressing different combinations of wild-type and OP-CDV glycoproteins. We show that low fusogenicity is not due to a defect of the envelope proteins to reach the cell surface and that H(WT) determines persistent infection in a receptor-dependent manner, emphasizing the role of SLAM as a potent enhancer of fusogenicity. However, importantly, F(WT) reduced cell-to-cell fusion independently of the cell surface receptor, thus demonstrating that the fusion protein of the neurovirulent A75/17-CDV strain plays a key role in determining persistent infection.
Keywords
Amino Acids/analysis, Animals, Biotinylation, Cercopithecus aethiops, Distemper/virology, Distemper Virus, Canine/pathogenicity, Dogs, Fluorescent Antibody Technique, Indirect, Transfection, Vero Cells, Viral Fusion Proteins/analysis, Viral Fusion Proteins/chemistry
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 10:43
Last modification date
20/08/2019 16:10
Usage data