MR evaluation of the glomerular homing of magnetically labeled mesenchymal stem cells in a rat model of nephropathy.

Details

Serval ID
serval:BIB_E63C88B56795
Type
Article: article from journal or magazin.
Collection
Publications
Title
MR evaluation of the glomerular homing of magnetically labeled mesenchymal stem cells in a rat model of nephropathy.
Journal
Radiology
Author(s)
Hauger O., Frost E.E., van Heeswijk R., Deminière C., Xue R., Delmas Y., Combe C., Moonen C.T., Grenier N., Bulte J.W.
ISSN
0033-8419 (Print)
ISSN-L
0033-8419
Publication state
Published
Issued date
2006
Volume
238
Number
1
Pages
200-210
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
PURPOSE: To assess renal glomerular homing of intravenously injected superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs) at in vivo and ex vivo magnetic resonance (MR) imaging in an experimental rat model of mesangiolysis.
MATERIALS AND METHODS: Animal procedures were performed in accordance with protocols approved by Institutional Animal Care and Use Committee. Fourteen rats were divided into two groups: one pathologic (n = 10), with persistent mesangiolysis following simultaneous injection of OX-7 monoclonal antibody and puromycin aminonucleoside in which 10(7) SPIO- and DiI-labeled MSCs were injected, and one control (n = 4). In vivo and ex vivo MR imaging examinations were performed with 4.7- and 9.4-T spectrometers, respectively, and T2*-weighted sequences. In vivo signal intensity variations were measured in the liver and kidney before and 6 days after MSC injection. Intrarenal signal intensity variations were correlated with histopathologic data by means of colocalization of DiI fluorescence, alpha-actin, and Prussian blue stain-positive cells. Histologic differences between the glomerular homing of MSCs in different kidney portions were correlated to the areas of MR signal intensity decrease with nonparametric statistical tests.
RESULTS: On in vivo images, signal intensity measurements of pathologic kidneys following MSC injection did not show any signal intensity decrease (P = .7), whereas a 34% +/- 14 (mean +/- standard deviation) signal intensity decrease was observed in the liver (P < .01), where a substantial number of labeled cells were trapped. On ex vivo images, pathologic kidneys showed focal cortical (glomerular) areas of signal intensity loss, which was absent in controls. The areas of low signal intensity correlated well with alpha-actin and Prussian blue stain- and DiI-positive areas (P < .01), which indicates that MSCs specifically home to injured tissue. No MSCs were detected in the kidneys of control animals.
CONCLUSION: Intravenously injected MSCs specifically home to focal areas of glomerular damage and can be detected at ex vivo MR imaging.
Keywords
Animals, Cell Culture Techniques, Contrast Media, Dextrans, Ferumoxytol, Image Processing, Computer-Assisted, Immunohistochemistry, Iron/diagnostic use, Kidney Diseases/pathology, Kidney Diseases/therapy, Kidney Glomerulus/cytology, Magnetic Resonance Imaging/methods, Magnetite Nanoparticles, Male, Mesenchymal Stem Cell Transplantation/methods, Oxides/diagnostic use, Rats, Rats, Inbred Lew, Staining and Labeling
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13/12/2012 16:31
Last modification date
20/08/2019 16:09
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