MR evaluation of the glomerular homing of magnetically labeled mesenchymal stem cells in a rat model of nephropathy.

Détails

ID Serval
serval:BIB_E63C88B56795
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
MR evaluation of the glomerular homing of magnetically labeled mesenchymal stem cells in a rat model of nephropathy.
Périodique
Radiology
Auteur⸱e⸱s
Hauger O., Frost E.E., van Heeswijk R., Deminière C., Xue R., Delmas Y., Combe C., Moonen C.T., Grenier N., Bulte J.W.
ISSN
0033-8419 (Print)
ISSN-L
0033-8419
Statut éditorial
Publié
Date de publication
2006
Volume
238
Numéro
1
Pages
200-210
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
PURPOSE: To assess renal glomerular homing of intravenously injected superparamagnetic iron oxide (SPIO)-labeled mesenchymal stem cells (MSCs) at in vivo and ex vivo magnetic resonance (MR) imaging in an experimental rat model of mesangiolysis.
MATERIALS AND METHODS: Animal procedures were performed in accordance with protocols approved by Institutional Animal Care and Use Committee. Fourteen rats were divided into two groups: one pathologic (n = 10), with persistent mesangiolysis following simultaneous injection of OX-7 monoclonal antibody and puromycin aminonucleoside in which 10(7) SPIO- and DiI-labeled MSCs were injected, and one control (n = 4). In vivo and ex vivo MR imaging examinations were performed with 4.7- and 9.4-T spectrometers, respectively, and T2*-weighted sequences. In vivo signal intensity variations were measured in the liver and kidney before and 6 days after MSC injection. Intrarenal signal intensity variations were correlated with histopathologic data by means of colocalization of DiI fluorescence, alpha-actin, and Prussian blue stain-positive cells. Histologic differences between the glomerular homing of MSCs in different kidney portions were correlated to the areas of MR signal intensity decrease with nonparametric statistical tests.
RESULTS: On in vivo images, signal intensity measurements of pathologic kidneys following MSC injection did not show any signal intensity decrease (P = .7), whereas a 34% +/- 14 (mean +/- standard deviation) signal intensity decrease was observed in the liver (P < .01), where a substantial number of labeled cells were trapped. On ex vivo images, pathologic kidneys showed focal cortical (glomerular) areas of signal intensity loss, which was absent in controls. The areas of low signal intensity correlated well with alpha-actin and Prussian blue stain- and DiI-positive areas (P < .01), which indicates that MSCs specifically home to injured tissue. No MSCs were detected in the kidneys of control animals.
CONCLUSION: Intravenously injected MSCs specifically home to focal areas of glomerular damage and can be detected at ex vivo MR imaging.
Mots-clé
Animals, Cell Culture Techniques, Contrast Media, Dextrans, Ferumoxytol, Image Processing, Computer-Assisted, Immunohistochemistry, Iron/diagnostic use, Kidney Diseases/pathology, Kidney Diseases/therapy, Kidney Glomerulus/cytology, Magnetic Resonance Imaging/methods, Magnetite Nanoparticles, Male, Mesenchymal Stem Cell Transplantation/methods, Oxides/diagnostic use, Rats, Rats, Inbred Lew, Staining and Labeling
Pubmed
Création de la notice
13/12/2012 16:31
Dernière modification de la notice
20/08/2019 16:09
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