SLP-2 interacts with prohibitins in the mitochondrial inner membrane and contributes to their stability.

Details

Serval ID
serval:BIB_E24D37BB1394
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
SLP-2 interacts with prohibitins in the mitochondrial inner membrane and contributes to their stability.
Journal
Biochimica et Biophysica Acta
Author(s)
Da Cruz S., Parone P.A., Gonzalo P., Bienvenut W.V., Tondera D., Jourdain A., Quadroni M., Martinou J.C.
ISSN
0167-4889
Publication state
Published
Issued date
02/2008
Peer-reviewed
Oui
Volume
1783
Number
5
Pages
904-911
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Stomatin is a member of a large family of proteins including prohibitins, HflK/C, flotillins, mechanoreceptors and plant defense proteins, that are thought to play a role in protein turnover. Using different proteomic approaches, we and others have identified SLP-2, a member of the stomatin gene family, as a component of the mitochondria. In this study, we show that SLP-2 is strongly associated with the mitochondrial inner membrane and that it interacts with prohibitins. Depleting HeLa cells of SLP-2 lead to increased proteolysis of prohibitins and of subunits of the respiratory chain complexes I and IV. Further supporting the role of SLP-2 in regulating the stability of specific mitochondrial proteins, we found that SLP-2 is up-regulated under conditions of mitochondrial stress leading to increased protein turnover. These data indicate that SLP-2 plays a role in regulating the stability of mitochondrial proteins including prohibitins and subunits of respiratory chain complexes.
Keywords
Animals, Blood Proteins/metabolism, Cells, Cultured, Electron Transport Complex I/metabolism, Electron Transport Complex IV/metabolism, HeLa Cells, Humans, Immunoprecipitation, Membrane Proteins/metabolism, Mitochondria/metabolism, Mitochondrial Membranes/metabolism, Mitochondrial Proteins/metabolism, Repressor Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
17/03/2008 10:08
Last modification date
20/08/2019 17:06
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