Combined use of fluorescent reporters and flow cytometry for simultaneous monitoring of bacterial growth and gene expression on plant roots
Details
Serval ID
serval:BIB_E10BD8E0B142
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
Combined use of fluorescent reporters and flow cytometry for simultaneous monitoring of bacterial growth and gene expression on plant roots
Title of the book
Handbook of hydrocarbon and lipid microbiology
Publisher
Springer
Address of publication
Berlin
ISBN
978-3-540-77584-3
Publication state
Published
Issued date
2010
Editor
Timmis K. N.
Volume
5
Series
Springer Reference
Chapter
7
Pages
3461-3470
Language
english
Notes
A protocol of a recently developed assay is presented in which FACS-based flow cytometry is used in combination with autofluorescent reporter constructs to simultaneously monitor and quantify bacterial growth (root colonization levels) and single cell activity (expression of a plant-beneficial trait) on roots of hydroponically grown plants. The experimental approach is illustrated with an example of a reporter of antifungal gene expression in a plant-beneficial strain of Pseudomonas fluorescens. It can be adapted to the study of other bacteria-root interactions.
Create date
04/03/2009 17:05
Last modification date
20/08/2019 17:05