Recovery of a persistent Canine distemper virus expressing the enhanced green fluorescent protein from cloned cDNA.

Details

Serval ID
serval:BIB_E09120ADE622
Type
Article: article from journal or magazin.
Collection
Publications
Title
Recovery of a persistent Canine distemper virus expressing the enhanced green fluorescent protein from cloned cDNA.
Journal
Virus Research
Author(s)
Plattet P., Zweifel C., Wiederkehr C., Belloy L., Cherpillod P., Zurbriggen A., Wittek R.
ISSN
0168-1702[print], 0168-1702[linking]
Publication state
Published
Issued date
05/2004
Volume
101
Number
2
Pages
147-153
Language
english
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Wild-type A75/17-Canine distemper virus (CDV) is a highly virulent strain, which induces a persistent infection in the central nervous system (CNS) with demyelinating disease. Wild-type A75/17-CDV, which is unable to replicate in cell lines to detectable levels, was adapted to grow in Vero cells and was designated A75/17-V. Sequence comparison between the two genomes revealed seven nucleotide differences located in the phosphoprotein (P), the matrix (M) and the large (L) genes. The P gene is polycistronic and encodes two auxiliary proteins, V and C, besides the P protein. The mutations resulted in amino acid changes in the P and V, but not in the C protein, as well as in the M and L proteins. Here, a rescue system was developed for the A75/17-V strain, which was shown to be attenuated in vivo, but retains a persistent infection phenotype in Vero cells. In order to track the recombinant virus, an additional transcription unit coding for the enhanced green fluorescent protein (eGFP) was inserted at the 3' proximal position in the A75/17-V cDNA clone. Reverse genetics technology will allow us to characterize the genetic determinants of A75/17-V CDV persistent infection in cell culture.
Keywords
Adaptation, Physiological, Amino Acid Substitution, Animals, Cell Line, Cercopithecus aethiops, Cloning, Molecular, Distemper Virus, Canine/genetics, Distemper Virus, Canine/growth &amp, development, Dogs, Gene Order, Genes, Reporter, Genes, Viral, Genome, Viral, Genomics, Green Fluorescent Proteins, Luminescent Proteins/genetics, Luminescent Proteins/metabolism, Mutation, Missense, Recombination, Genetic, Vero Cells, Viral Proteins/chemistry, Viral Proteins/genetics
Pubmed
Web of science
Create date
24/01/2008 11:43
Last modification date
20/08/2019 17:04
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