Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains.

Details

Serval ID
serval:BIB_DD71DF1076BB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains.
Journal
Journal of Clinical Microbiology
Author(s)
Bizzini A., Jaton K., Romo D., Bille J., Prod'hom G., Greub G.
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Publication state
Published
Issued date
2011
Volume
49
Number
2
Pages
693-696
Language
english
Abstract
Conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16S rRNA gene sequencing. MALDI-TOF MS might represent an alternative to 16S rRNA gene sequencing.
Keywords
routine identification, sp nov., microbiology, pcr
Pubmed
Web of science
Open Access
Yes
Create date
31/03/2011 13:33
Last modification date
20/08/2019 16:02
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