Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains.
Détails
ID Serval
serval:BIB_DD71DF1076BB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Matrix-assisted laser desorption ionization-time of flight mass spectrometry as an alternative to 16S rRNA gene sequencing for identification of difficult-to-identify bacterial strains.
Périodique
Journal of Clinical Microbiology
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Statut éditorial
Publié
Date de publication
2011
Volume
49
Numéro
2
Pages
693-696
Langue
anglais
Résumé
Conventional methods are sometimes insufficient to identify human bacterial pathogens, and alternative techniques, often molecular, are required. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identified with a valid score 45.9% of 410 clinical isolates from 207 different difficult-to-identify species having required 16S rRNA gene sequencing. MALDI-TOF MS might represent an alternative to 16S rRNA gene sequencing.
Mots-clé
routine identification, sp nov., microbiology, pcr
Pubmed
Web of science
Open Access
Oui
Création de la notice
31/03/2011 14:33
Dernière modification de la notice
20/08/2019 17:02
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