Sequence microheterogeneity in apolipoprotein(a) gene repeats and the relationship to plasma Lp(a) levels.

Details

Serval ID
serval:BIB_DD462A404A99
Type
Article: article from journal or magazin.
Collection
Publications
Title
Sequence microheterogeneity in apolipoprotein(a) gene repeats and the relationship to plasma Lp(a) levels.
Journal
Human Molecular Genetics
Author(s)
Mancini F.P., Mooser V., Guerra R., Hobbs H.H.
ISSN
0964-6906 (Print)
ISSN-L
0964-6906
Publication state
Published
Issued date
1995
Volume
4
Number
9
Pages
1535-1542
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't,
Abstract
Lipoprotein(a) [Lp(a)] is a cholesterol ester-rich atherogenic lipoprotein that is composed of a particle of low density lipoprotein and a large glycoprotein, apolipoprotein(a) [apo(a)]. Apolipoprotein(a) varies in size over a approximately 500 kDa range due to inter-allelic differences in the number of tandemly repeated kringle 4 (K4)-encoding 5.5 kb sequences in the apo(a) gene. Only one of the 10 different types of K4 repeats in the apo(a) gene, the so-called type 2 K4 repeats, vary in number between apo(a) alleles. In this paper, we show that there is microheterogeneity within the sequence of the type 2 K4 repeat. DraIII restriction digestion and genomic blotting revealed that a subset of the type 2 K4-encoding sequences contained a DraIII site (K4-D). The proportion of apo(a) alleles that had at least one K4-D repeat ranged from 25% in Caucasians to 50% in the Chinese. K4-D repeats were clustered at the end(s) of the type 2 K4 tandem array and the number and patterns of the K4-D repeats were in linkage disequilibrium with flanking sequence polymorphisms; these features are remarkably similar to the minisatellite variant repeats (MVRs) found in variable number of tandem repeat sequences (VNTRs). In addition, a DraIII pattern that comprised 9% of the sample was invariably associated with low plasma levels of Lp(a) in Caucasians.
Keywords
Apolipoproteins/genetics, Apoprotein(a), Base Sequence, DNA, Deoxyribonucleases, Type II Site-Specific, Ethnic Groups/genetics, Humans, Lipoprotein(a)/blood, Molecular Sequence Data, Polymorphism, Genetic, Repetitive Sequences, Nucleic Acid
Pubmed
Web of science
Create date
13/10/2013 21:06
Last modification date
20/08/2019 16:02
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