Sequence microheterogeneity in apolipoprotein(a) gene repeats and the relationship to plasma Lp(a) levels.
Details
Serval ID
serval:BIB_DD462A404A99
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sequence microheterogeneity in apolipoprotein(a) gene repeats and the relationship to plasma Lp(a) levels.
Journal
Human Molecular Genetics
ISSN
0964-6906 (Print)
ISSN-L
0964-6906
Publication state
Published
Issued date
1995
Volume
4
Number
9
Pages
1535-1542
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't,
Abstract
Lipoprotein(a) [Lp(a)] is a cholesterol ester-rich atherogenic lipoprotein that is composed of a particle of low density lipoprotein and a large glycoprotein, apolipoprotein(a) [apo(a)]. Apolipoprotein(a) varies in size over a approximately 500 kDa range due to inter-allelic differences in the number of tandemly repeated kringle 4 (K4)-encoding 5.5 kb sequences in the apo(a) gene. Only one of the 10 different types of K4 repeats in the apo(a) gene, the so-called type 2 K4 repeats, vary in number between apo(a) alleles. In this paper, we show that there is microheterogeneity within the sequence of the type 2 K4 repeat. DraIII restriction digestion and genomic blotting revealed that a subset of the type 2 K4-encoding sequences contained a DraIII site (K4-D). The proportion of apo(a) alleles that had at least one K4-D repeat ranged from 25% in Caucasians to 50% in the Chinese. K4-D repeats were clustered at the end(s) of the type 2 K4 tandem array and the number and patterns of the K4-D repeats were in linkage disequilibrium with flanking sequence polymorphisms; these features are remarkably similar to the minisatellite variant repeats (MVRs) found in variable number of tandem repeat sequences (VNTRs). In addition, a DraIII pattern that comprised 9% of the sample was invariably associated with low plasma levels of Lp(a) in Caucasians.
Keywords
Apolipoproteins/genetics, Apoprotein(a), Base Sequence, DNA, Deoxyribonucleases, Type II Site-Specific, Ethnic Groups/genetics, Humans, Lipoprotein(a)/blood, Molecular Sequence Data, Polymorphism, Genetic, Repetitive Sequences, Nucleic Acid
Pubmed
Web of science
Create date
13/10/2013 22:06
Last modification date
20/08/2019 17:02