Ten years of R&D and full automation in molecular diagnosis.

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State: Public
Version: Final published version
Serval ID
serval:BIB_DD3A1054F7C7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ten years of R&D and full automation in molecular diagnosis.
Journal
Future Microbiology
Author(s)
Greub G., Sahli R., Brouillet R., Jaton K.
ISSN
1746-0921 (Electronic)
ISSN-L
1746-0913
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
11
Pages
403-425
Language
english
Abstract
A 10-year experience of our automated molecular diagnostic platform that carries out 91 different real-time PCR is described. Progresses and future perspectives in molecular diagnostic microbiology are reviewed: why automation is important; how our platform was implemented; how homemade PCRs were developed; the advantages/disadvantages of homemade PCRs, including the critical aspects of troubleshooting and the need to further reduce the turnaround time for specific samples, at least for defined clinical settings such as emergencies. The future of molecular diagnosis depends on automation, and in a novel perspective, it is time now to fully acknowledge the true contribution of molecular diagnostic and to reconsider the indication for PCR, by also using these tests as first-line assays.
Pubmed
Web of science
Open Access
Yes
Create date
09/04/2016 16:05
Last modification date
20/08/2019 17:02
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