Clonal expansion of T cells: a cytotoxic T-cell response in vivo that involves precursor cell proliferation.

Details

Serval ID
serval:BIB_DCC3C2E897DC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Clonal expansion of T cells: a cytotoxic T-cell response in vivo that involves precursor cell proliferation.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Denizot F., Wilson A., Battye F., Berke G., Shortman K.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Publication state
Published
Issued date
1986
Volume
83
Number
16
Pages
6089-6092
Language
english
Abstract
The response of peritoneal exudate lymphocytes to allogeneic tumor cells was used to determine whether the in vivo generation of cytotoxic T cells (CTL) involved the proliferation of precursor cells. Ten days post-injection, both cytotoxic activity and the formation of conjugates between lymphocytes and target cells were shown to be specific for the immunizing tumor alloantigens and to be effected by Ly-2+ cells. A cell-sorting-based procedure was developed to isolate specific conjugates between red-fluorescence-tagged CTL and blue-fluorescence-tagged tumor target cells. When [3H]thymidine was administered during the response, almost all isolated conjugate-forming CTL were 3H-labeled on autoradiography. Thus, the CTL were clearly products of dividing cells, a result that contradicts published data. Reassessment of a previously studied system, which suggested that CTL were not products of cell division, indicated that in that system many of the conjugate-forming cytotoxic cells studied were Ly-2- and nonspecific, and thus perhaps not T cells. We conclude that the clonal selection model is applicable to at least one in vivo T-cell response.
Keywords
Animals, Cell Division, Cell Line, Clone Cells, Cytotoxicity, Immunologic, DNA Replication, Lymphocyte Activation, Lymphoma/immunology, Mast-Cell Sarcoma/immunology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred CBA, Phenotype, T-Lymphocytes, Cytotoxic/immunology, Thymidine/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
10/04/2013 12:41
Last modification date
20/08/2019 16:01
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