Clonal expansion of T cells: a cytotoxic T-cell response in vivo that involves precursor cell proliferation.
Details
Serval ID
serval:BIB_DCC3C2E897DC
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Clonal expansion of T cells: a cytotoxic T-cell response in vivo that involves precursor cell proliferation.
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Publication state
Published
Issued date
1986
Volume
83
Number
16
Pages
6089-6092
Language
english
Abstract
The response of peritoneal exudate lymphocytes to allogeneic tumor cells was used to determine whether the in vivo generation of cytotoxic T cells (CTL) involved the proliferation of precursor cells. Ten days post-injection, both cytotoxic activity and the formation of conjugates between lymphocytes and target cells were shown to be specific for the immunizing tumor alloantigens and to be effected by Ly-2+ cells. A cell-sorting-based procedure was developed to isolate specific conjugates between red-fluorescence-tagged CTL and blue-fluorescence-tagged tumor target cells. When [3H]thymidine was administered during the response, almost all isolated conjugate-forming CTL were 3H-labeled on autoradiography. Thus, the CTL were clearly products of dividing cells, a result that contradicts published data. Reassessment of a previously studied system, which suggested that CTL were not products of cell division, indicated that in that system many of the conjugate-forming cytotoxic cells studied were Ly-2- and nonspecific, and thus perhaps not T cells. We conclude that the clonal selection model is applicable to at least one in vivo T-cell response.
Keywords
Animals, Cell Division, Cell Line, Clone Cells, Cytotoxicity, Immunologic, DNA Replication, Lymphocyte Activation, Lymphoma/immunology, Mast-Cell Sarcoma/immunology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred CBA, Phenotype, T-Lymphocytes, Cytotoxic/immunology, Thymidine/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
10/04/2013 12:41
Last modification date
20/08/2019 16:01