Tomographic in vivo microscopy for the study of lung physiology at the alveolar level.

Details

Ressource 1Download: 28970505_BIB_DB03D8E933E8.pdf (5714.07 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_DB03D8E933E8
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tomographic in vivo microscopy for the study of lung physiology at the alveolar level.
Journal
Scientific reports
Author(s)
Lovric G., Mokso R., Arcadu F., Vogiatzis Oikonomidis I., Schittny J.C., Roth-Kleiner M., Stampanoni M.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
02/10/2017
Peer-reviewed
Oui
Volume
7
Number
1
Pages
12545
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Lungs represent the essential part of the mammalian respiratory system, which is reflected in the fact that lung failure still is one of the leading causes of morbidity and mortality worldwide. Establishing the connection between macroscopic observations of inspiration and expiration and the processes taking place at the microscopic scale remains crucial to understand fundamental physiological and pathological processes. Here we demonstrate for the first time in vivo synchrotron-based tomographic imaging of lungs with pixel sizes down to a micrometer, enabling first insights into high-resolution lung structure. We report the methodological ability to study lung inflation patterns at the alveolar scale and its potential in resolving still open questions in lung physiology. As a first application, we identified heterogeneous distension patterns at the alveolar level and assessed first comparisons of lungs between the in vivo and immediate post mortem states.
Keywords
Animals, Autopsy, Humans, Intravital Microscopy/methods, Lung/diagnostic imaging, Lung/physiopathology, Lung/ultrastructure, Pulmonary Alveoli/diagnostic imaging, Pulmonary Alveoli/ultrastructure, Rats, Respiratory Distress Syndrome, Adult/diagnostic imaging, Respiratory Distress Syndrome, Adult/physiopathology, Respiratory Physiological Phenomena, Tomography, X-Ray Computed/methods
Pubmed
Web of science
Open Access
Yes
Create date
09/10/2017 14:34
Last modification date
20/08/2019 17:00
Usage data