Tumor and non-tumor liver angiogenesis is traced and evaluated by hepatic arterial ultrasound in murine models.
Details
Serval ID
serval:BIB_DAE2A97FC69E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Tumor and non-tumor liver angiogenesis is traced and evaluated by hepatic arterial ultrasound in murine models.
Journal
Ultrasound In Medicine and Biology
ISSN
1879-291X (Electronic)
ISSN-L
0301-5629
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
38
Number
7
Pages
1195-1204
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
We studied the relationships between hepatic and mesenteric mean blood-flow velocities (mBFVs) measured by ultrasound imaging and (1) downstream tumor angiogenesis during liver metastasis induced by spleen injection of LS174 human colon cells overexpressing the antiangiogenic Netrin4 (LS174-NT4) or not (LS174-WT) and (2) downstream normal angiogenesis during hepatic regeneration after 50% hepatectomy. Liver volume and mBFVs were measured before and after surgery, at day 30 in the first model and at days 2, 7 and 16 in the second model. LS174-NT-4 vs. LS174-WT mice presented fewer metastases (25% vs. 90%, p < 0.001) and decreased hepatic mBFVs (16.5 ± 0.8 vs. 21.8 ± 1.4 cm s(-1), p < 0.01), without difference in mesenteric mBFVs. After partial hepatectomy, hepatic and mesenteric mBFVs increased at day 7, from 12.4 ± 1.7 and 11.8 ± 2.6 to 19.1 ± 1.8 and 17.5 ± 2.4 cm s(-1), respectively, (p < 0.01) then returned to baseline as liver volume. Duplex Doppler ultrasonography reliably assesses normal or tumor angiogenesis and may provide follow-up functional evaluation.
Keywords
Animals, Cell Line, Tumor, Disease Models, Animal, Hepatic Artery/ultrasonography, Humans, Liver Neoplasms/complications, Liver Neoplasms/ultrasonography, Mice, Neovascularization, Pathologic/ultrasonography, Reproducibility of Results, Sensitivity and Specificity
Pubmed
Web of science
Create date
13/10/2015 11:14
Last modification date
20/08/2019 17:00