Tumor and non-tumor liver angiogenesis is traced and evaluated by hepatic arterial ultrasound in murine models.

Details

Serval ID
serval:BIB_DAE2A97FC69E
Type
Article: article from journal or magazin.
Collection
Publications
Title
Tumor and non-tumor liver angiogenesis is traced and evaluated by hepatic arterial ultrasound in murine models.
Journal
Ultrasound In Medicine and Biology
Author(s)
Eveno C., Le Henaff C., Audollent R., Soyer P., Rampanou A., Nemeth J., Brouland J.P., Dupuy E., Pocard M., Bonnin P.
ISSN
1879-291X (Electronic)
ISSN-L
0301-5629
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
38
Number
7
Pages
1195-1204
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
We studied the relationships between hepatic and mesenteric mean blood-flow velocities (mBFVs) measured by ultrasound imaging and (1) downstream tumor angiogenesis during liver metastasis induced by spleen injection of LS174 human colon cells overexpressing the antiangiogenic Netrin4 (LS174-NT4) or not (LS174-WT) and (2) downstream normal angiogenesis during hepatic regeneration after 50% hepatectomy. Liver volume and mBFVs were measured before and after surgery, at day 30 in the first model and at days 2, 7 and 16 in the second model. LS174-NT-4 vs. LS174-WT mice presented fewer metastases (25% vs. 90%, p < 0.001) and decreased hepatic mBFVs (16.5 ± 0.8 vs. 21.8 ± 1.4 cm s(-1), p < 0.01), without difference in mesenteric mBFVs. After partial hepatectomy, hepatic and mesenteric mBFVs increased at day 7, from 12.4 ± 1.7 and 11.8 ± 2.6 to 19.1 ± 1.8 and 17.5 ± 2.4 cm s(-1), respectively, (p < 0.01) then returned to baseline as liver volume. Duplex Doppler ultrasonography reliably assesses normal or tumor angiogenesis and may provide follow-up functional evaluation.
Keywords
Animals, Cell Line, Tumor, Disease Models, Animal, Hepatic Artery/ultrasonography, Humans, Liver Neoplasms/complications, Liver Neoplasms/ultrasonography, Mice, Neovascularization, Pathologic/ultrasonography, Reproducibility of Results, Sensitivity and Specificity
Pubmed
Web of science
Create date
13/10/2015 10:14
Last modification date
20/08/2019 16:00
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