A monoclonal antibody against altered LFA-1 induces proliferation and lymphokine release of cloned T cells.
Details
Serval ID
serval:BIB_DA0D161A6DD2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A monoclonal antibody against altered LFA-1 induces proliferation and lymphokine release of cloned T cells.
Journal
European Journal of Immunology
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Publication state
Published
Issued date
1986
Volume
16
Number
2
Pages
172-181
Language
english
Abstract
A murine monoclonal antibody (I-17, IgM) has the following functional effects on murine long-term T cell clones: inhibition of cell-mediated lysis, induction of proliferation, release of lymphokines and change of the cell morphology. The determinant detected by I-17 is expressed on long-term T lines but not on thymocytes, lymph node cells and spleen cells. I-17 precipitated proteins with apparent molecular mass of 220 kDa, 170 kDa, 150 kDa and 100 kDa. Biochemical studies indicate that the determinant recognized by I-17 is tunicamycin sensitive and that I-17 binds to the alpha chain of the lymphocyte function-associated antigen (LFA-1).
Keywords
Animals, Antibodies, Monoclonal/biosynthesis, Antibodies, Monoclonal/immunology, Antigens, Surface/analysis, Antigens, Surface/immunology, Cell Adhesion, Chemical Precipitation, Clone Cells, Concanavalin A/pharmacology, Cytotoxicity, Immunologic, Epitopes/analysis, Female, Lymphocyte Activation, Lymphocyte Function-Associated Antigen-1, Lymphokines/secretion, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Protein Conformation, T-Lymphocytes/immunology, T-Lymphocytes, Cytotoxic/ultrastructure, Tunicamycin/pharmacology
Pubmed
Web of science
Create date
28/01/2008 12:36
Last modification date
20/08/2019 16:59
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